Origin and direction of replication of the drug resistance plasmid R100.1 and of a resistance transfer factor derivative in synchronized cultures.

نویسندگان

  • L Silver
  • M Chandler
  • E B de la Tour
  • L Caro
چکیده

The origin and direction of replication of the resistance plasmid R100.1 and its resistance transfer factor derivative, pAR132, were studied by electron microscopy autoradiography of partially denatured molecules and partial denaturation mapping of replicative intermediates. Results of these studies indicate the existence of an origin of replication at 8.8 kilobases on the R100 map. Replication from this origin in cultures synchronized for initiation of replication is predominantly unidirectional in a single direction.

برای دانلود رایگان متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

The evaluation of phenotyping and molecular resistance to antibiotics in Proteus species isolated from urinary tract infections in Ilam city

Introduction: Resistance of pathogenic organisms to countenance antibiotics has become a worldwide problem with serious consequences on the treatment of infectious diseases. The aim of this study was to evaluate antibiotic resistance and also the detection of transferred antibiotic resistance by plasmid in clinical Proteus isolates. Materials and methods: A total of 250 urine samples were ...

متن کامل

Plasmid- mediated cefotaxime and ceftizoximd resistance in Ten strains of klebsiella pneumoniae isolated from hospitals in Kerman,Iran

Klebsiella pneumonia is a gram negative bacillus of which it's resistant strains are a common source of nosocomial infections . The goal of this study is determination of drug sensitivity of these resistant strains, and study of causes involved in the resistant especially the presence of plasmid. Ten different strains of klebsiella pneumoniae were isolated from clinical specimens collected from...

متن کامل

Evaluation of Virulence Factors and Plasmid-Related Transmissibility among Different Isolates of Enterococci

The incidence of virulence factors among 114 Enterococcus faecalis and 35 Enterococcus faecium strains from different clinical specimens were compared with those isolated from control groups. A few of the isolates expressed two or more of the following traits simultaneously: hemolysin, aggregation substance, gelatinase, DNase, hemagglutinin and antibiotic resistance. The frequencies of hemolysi...

متن کامل

انجام آمیختگی مصنوعی در باکتری اشریشیاکلی جهت بررسی انتقال پلاسمید مولد پنی سیلیناز

Conjugation is a major mechanism of DNA transfer among bacteria which takes place in both laboratory and natural environment and brings about new characteristics. Extrachromosomal DNA called plasmid are important elements in this exchange. Resistance plasmid confer resistance to many antibiotics and in this group, penicillinase plasmid is responsible for resistance to penicillins and cephalos...

متن کامل

Varicella Zoster Virus (VZV) Origin-Dependent Plasmid Replication in the Presence of the Four Overlapping Cosmids Comprising the Complete Genome of VZV

The Varicella-Zoster Virus (VZV) genome contains both cis-acting and trans-acting elements, which are important in viral DNA replication. The cis-acting elements consist of two copies of oriS, and the trans-acting elements are those genes whose products are required for virus DNA replication. It has been shown that each of the seven genes required for ori-dependent DNA synthesis of Herpes Simpl...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

عنوان ژورنال:
  • Journal of bacteriology

دوره 131 3  شماره 

صفحات  -

تاریخ انتشار 1977